pipette 25 c.c. Benedict's solution into a porcelain
dish, add 5 or 10 grams approximately of solid sodic carbonate, heat
to boiling, and while boiling, run in urine until a white precipitate
forms, then add urine more slowly until the last trace of blue
disappears. The urine should be diluted so that not less than 10 c.c.
will be required to amount of sugar which 25 c.c. of reagent is
capable of oxidizing.

Calculation: 5 divided by number of cubic centimeters of urine run in
equals per cent. of sugar.

~Fermentation Test for Quantity of Sugar in Urine.~--If the urine is
70 deg. F. (room) temperature when the specific gravity is taken at both
the beginning and end of the test, it will assure accuracy.

To 100 c.c. of urine of known specific gravity, add one-fourth of
fresh yeast cake thoroughly broken up. Mix thoroughly and set aside at
a temperature between 85 deg. and 95 deg. F. for twenty-four hours, after
which time test with Benedict's or Fehling's solutions. If reduction
is obtained, it will be necessary to allow the fermentation to
continue until it is complete. When no further reduction is obtained,
the specific gravity is taken after the urine has reached a
temperature of 76 deg.. The difference in the specific gravity at the
beginning and end of the test multiplied by 0.23 gives the percentage
of sugar in the urine.

The following formulas represent the various solutions used in the
above test:

BENEDICT'S QUALITATIVE SOLUTION

_Gm. or c.c._
Copper sulphate (pure crystals) 17.3
Sodium or potassium citrate 173.0
Sodium carbonate (anhydrous) 100.0
Distilled water to make 1000.0

FEHLING'S SOLUTION

(1) Copper Sulphate Solution:
34.65 grams copper sulphate dissolved in water and sufficient
water added to make 500 c.c.
(2) Alkaline Solution:
125 grams potassium hydroxide.
173 grams Rochelle salts dissolved in water q.s. to make 500 c.c.

Keep solution in separate bottles and mix in equal quantities when
ready to use.

HAINES'S SOLUTION

Copper sulphate (pure) 30 grams
(dissolved in 1/2 oz. (15 c.c.) distilled water)

Add 1/2 oz. pure glycerin, mix thoroughly, and add 5 oz. liquor
potassae.

BENEDICT'S (QUANTITATIVE) SOLUTION

Copper sulphate (pure crystals) 18 grams
Sodium carbonate (crystallized) (or 100