peritoneal
cavity (see Fig. 183).

[Illustration: FIG. 182.--Intraperitoneal inoculation--fluid.]

8. Inject the fluid from the syringe.

9. Label, etc.

[Illustration: FIG. 183.--Section of abdominal wall, etc., showing point
of needle lying free in the peritoneal cavity above the coils of
intestine.]

Second Method:

Steps 1-4. As in the first method.

5. Anaesthetise a small selected area of skin by spraying it with ethyl
chloride.

6. Heat platinum searing wire (0.5 mm. wire, twisted to the shape
indicated in figure 184, mounted in an aluminium handle) to redness, and
with it burn a hole through the anaesthetic area of skin and abdominal
muscle down to, but not through, the visceral peritoneum.

7. Fix a blunt-ended needle on to the charged syringe, and by pressing
the rounded end firmly against the peritoneum it can easily be pushed
through into the peritoneal cavity.

8. Inject the fluid from the syringe.

9. Label, etc.

This method is especially useful when it is desired to collect samples
of the peritoneal fluid from time to time during the period of
observation, as fluid can be removed from the peritoneal cavity, at
intervals, through this aperture in the abdominal parietes, by means of
a sterile capillary pipette.

[Illustration: FIG. 184.--Platinum wire for burning hole through
parietes.]

(b) _Solid Inoculum_ (or the implantation of capsules containing fluid
cultivations).--(_Anaesthetic, A. C. E._)

1. Anaesthetise the animal and secure it to the operating table.

2. Shave a large area of the abdominal parietes.

3. Make an incision through the skin in the middle line about 2 cm. in
length, midway between the lower end of the sternum and the pubes.

4. Divide the aponeuroses between the recti upon a director.

5. Divide the peritoneum upon a director.

6. Introduce the inoculum into the peritoneal cavity.

7. Close the peritoneal cavity with Lembert's sutures.

8. Close the skin and aponeurosis incisions together with interrupted
sutures or Michel's steel clips, and apply a sealed dressing.

9. Release the animal from the operating table.

10. Label, etc.

Suitable sacs may be readily prepared by either of the following
methods:

A. ~Collodion Sacs.~

1. Dip a small test-tube (5 by 0.5 cm.), bottom downward, into a beaker
of collodion, and dry in the air; repeat this process three or four
times.

2. Dip the tube, with its coating of collodion, alternately into a
beak