ile scissors and
a fresh pair of forceps, exposing the heart. Place the infected
instruments by the side of the first set.

11. Observe the condition of the anterior mediastinal glands, the thymus
and the lungs. Collect a quantity of pleuritic effusion, if such is
present, in a pipette for further examination later.

12. Raise the pericardial sac in a fresh pair of forceps and burn
through this structure with a searing iron.

Collect a sample of pericardial fluid in a pipette for microscopical and
cultural examination.

13. Grasp the apex of the heart in the forceps and sear the surface of
the right ventricle.

14. Plunge the open point of a capillary pipette through the seared area
into the ventricle and fill with blood.

Make cultivations and cover-slip preparations of the heart blood.

15. Collect a further sample of blood or serum for subsequent
investigation as to the presence of antibodies.

~Peritoneal Cavity.~--

16. Sear a broad track in the middle line of the abdominal wall; open
the peritoneal cavity by an incision in the centre of the seared line.
Observe the condition of the omentum, the mesentery, the viscera and the
peritoneal surface of the intestines.

17. Collect a specimen of the peritoneal fluid (or pus, if present) in a
capillary pipette. Make cultivations, tube and surface plate, and
cover-slip preparations from this situation.

18. Collect a specimen of the urine from the distended bladder in a
large pipette (in the manner indicated for heart blood), for further
examination, by cultivations, microscopical preparations, and chemical
analysis.

19. Collect a specimen of bile from the gall bladder in similar manner.

20. Excise the spleen and place it in a sterile capsule. Later, sear the
surface of this organ; plunge the spear-headed spatula through the
centre of the seared area, twist it round between the finger and thumb,
and remove it from the organ. Sufficient material will be brought away
in the eye in its head to make cultivations. A repetition of the process
will afford material for cover-slip preparations.

21. Seize one end of the spleen with sterile forceps. Sear a narrow band
of tissue, right around the organ and divide the spleen in this
situation with a pair of scissors. Holding the piece of spleen in the
forceps, dab the cut surface on to a surface plate in a number of
different spots.

22. In like manner examine the other organs--liver, lungs, kidneys,
lymphatic