with cosin.

6. Stain film preparation of pus from an abscess (containing
Staphylococcus pyogenes aureus) with carbolic methylene-blue and also by
Gram's method, counterstained with cosin.

7. Inoculate[15] a white mouse subcutaneously with three loopfuls of a
forty-eight-hour agar cultivation of the Staphylococcus aureus,
emulsified with 0.2 c.c. sterile broth.

Observe carefully during life, and when death occurs make a careful
post-mortem examination.

II. _Pyogenic cocci._

Micrococcus gonorrhoeae.
Micrococcus intracellularis meningitidis (meningococcus).
Micrococcus catarrhalis.
Micrococcus tetragenus.
Micrococcus paratetragenus.

III. _Pyogenic cocci._

Streptococcus pyogenes longus.
Streptococcus of bovine mastitis.
Streptococcus lanceolatus (Diplococcus pneumoniae or pneumococcus).
to contrast with
Streptococcus brevis.
Streptococcus lebensis.

IV. _Pyogenic bacilli._

Bacillus pneumoniae (Friedlaender).
Bacillus rhinoscleromatis.
Bacillus lactis aerogenes.

V. _Pyogenic bacilli._

Bacillus pyocyaneus.
to contrast with
Bacillus fluorescens liquefaciens.
Bacillus fluorescens non-liquefaciens.

VI. _Pneumonia group._

Streptococcus lanceolatus (pneumococcus).
Bacillus pneumoniae (Friedlaender).
Streptococcus pyogenes longus.

VII. _Diphtheroid group._

Bacillus diphtheriae (Klebs-Loeffler).
Bacillus Hoffmanni.
Bacillus xerosis.
Bacillus septus.

VIII. _Coli-typhoid group._

B. typhi abdominalis (B. typhosus).
B. coli communis.
B. enteritidis (Gaertner).
to contrast with
B. aquatilis sulcatus.

IX. _Escherich group._

B. coli communis (Escherich).
B. coli communior.
B. lactis aerogenes.
B. cloacae.

X. _Gaertner group._

Bacillus enteritidis (Gaertner).
B. paratyphosus A.
B. paratyphosus B.
Bacillus cholerae suum (Hog Cholera).
B. psittacosis.

XI. _Eberth group._

B. typhosus (Eberth).
B. dysenteriae (Shiga).
B. dysenteriae (Flexner).
B. faecalis alcaligines.

XII. _Spirillum group._

Vibrio cholerae.
Vibrio metschnikovi.
to contrast with
Vibrio proteus (Finkler and Prior).
Spirillum rubrum.
Spirillum rugula.

XIII. _Anthrax group._

Bacillus anthracis.
to contrast with
Bacillus subtilis.
Bacillus mycoides.
Bacillus mese