sterile water running
from an aspirator jar; deposit the oyster inside the sterile glass dish.
Repeat the process with each of the remaining oysters.

2. Before proceeding further, cleanse the hands thoroughly with clean
nail brush, soap and water, then plunge them in lysol 2 per cent.
solution, and finally in sterile water.

3. Spread a sterile towel on the bench.

4. Remove one of the oysters from the sterile glass dish and place it,
resting on its convex shell, on the towel. Turn a corner of the sterile
towel over the upper flat shell to give a firmer grip to the left hand,
which holds the shell in position.

5. With the sterile oyster knife (in the right hand) open the shell and
separate the body of the oyster from the inner surface of the upper flat
shell. Bend back and separate the flat shell, leaving the body of the
oyster in and attached to the concave shell. Avoid spilling any of the
liquor.

(Some dexterity in opening oysters should be acquired before undertaking
these experiments).

6. Cut up the body of the oyster with sterile scissors into small pieces
and allow the liquor freed from the body during the process to mix with
the liquor previously in the shell.

7. Transfer the comminuted oyster and the liquor to the cylinder.

8. Treat each of the remaining oysters in similar fashion.

9. Mix the contents of the cylinder thoroughly by stirring with a
sterile glass rod. The total volume will amount to about 100 c.c.

10. Use 0.1 c.c. of the mixed liquor to inseminate each of a series of
three nutrose surface plates.

11. Inoculate 0.1 c.c. of the mixed liquor into each of three tubes of
litmus milk.

12. Add sterile distilled water to the contents of the cylinder up to
1000 c.c. and stir thoroughly with a sterile glass rod and allow to
settle. The bacterial content of each oyster may be regarded, for all
practical purposes, as comprised in 100 c.c. of fluid.

13. Arrange four glass capsules in a row and number I, II, III, IV.
Pipette 9 c.c. sterile distilled water into each.

14. To capsule No. I add 1 c.c. of the diluted liquor, etc. from the
cylinder, and mix thoroughly. To capsule II add 1 c.c. of dilution in
capsule I and mix thoroughly. Carry over 1 c.c. of fluid from capsule
II to capsule III, afterwards adding 1 c.c. of fluid from capsule III to
capsule IV.

15. Label tubes of bile salt broth and inoculate with the following
amounts of diluted oysters:

No. 6 with 10 c.c. cylin