colonies, 262

Shaving experimental animals, 349

Shellfish, analysis of, 463

Silicate jelly (Winogradsky), 198

Single stain for spores, 106

Size of colonies, 262

Slanted tube cultures, 223

Slides, to clean new, 22
used, 23

Smear culture, 224
description of, 268

Soap liquid, 346

Soda solution, storage of stock, 154

Sodium bicarbonate in milk, test for, 443

Soil, analysis of, qualitative, 476
quantitative, 473
collection of samples, 471

Solid media, 147

Soluble toxins, 144

Soyka's milk rice, 189

Spear-headed spatula, 402

Special media, 182

Specific serum, 379
dilution of, 382

Spherical aberration, 55

Spirillum, morphology of, 133

Spirochaeta, morphology of, 133

Spirochaetes in tissues, to stain, 124

Spleen extract, 149

Sporangium, 127

Spore formation, arthrogenous, 138
endogenous, 138
method of, 138, 273
germination, method of, 140, 274
observation of, 140, 273

Spores, characters of, 139
classification of, 139
double stain for, 106
to stain, 106

Stab culture, 224
description of, 265

Stage micrometer, 62
of microscope, 52

Staining methods, 90
paraffin sections, 121
reactions of bacteria, 274

Stains intra-vitam, 77
negative (Burri), 77
rack for, 72

Standard graduated pipettes, 7
soda solution, 154

Standardisation of media, 154

Standardising bouillon, 155

Staphylococci, morphology, 132

Staphylococcus in milk, 456

Steam steriliser, Arnold, 35
Koch, 35
to use, 35
streaming, 35

Sterigma, 127

Sterilisation by chemicals, 27
by dry heat, 28
by filters, 40
by moist heat, 32
by streaming steam, 35
by superheated steam, 36
of albuminous liquids, 32
of gases, 40

Sterilising agents, 26

Stichcultur, 224

Stock dilutions, 497
nutrient media, 163
plate for isolation work, 253

Storage of media in bulk, 159
of tubed media, 161

Store boxes for media, 161

Streak culture, 224
description of, 268

Streaming movement, 80
steam, 35

Streptobacilli, morphology, 133

Streptococci in soil, 477
in water, detection of, 432
morphology of, 132

Streptococcus pyogenes longus in milk, 455

Streptothrix, morphology of, 133

Strichcultur, 223

Structure, internal, of colonies, 265

Study of pathogenic bacteria, 408

Subcutaneous inoculation, 353

Subdural inoculation, 361

Substage condenser, 54

Sugar agar, 185