dextrose bouillon, 184
gelatine, 184
media, preparation of, 177

Sulphindigotate agar, 181
bouillon (Weyl), 181
gelatine (Weyl), 181

Sulphuretted hydrogen in cultures, test for, 290

Sunlight, action of, 309

Superheated steam, 36

Superior lethal coefficient, 310, 313

Suppuration, organisms of, 409

Surface characters of colonies, 264
plates, 230

Surgical motor, electric, 360

Swarm spores, 127

Syringe for subcutaneous inoculation of solid material, 354
hypodermic, 344

Tatin's operating table, 351

Taxonomy, 262

Teat-pipettes, 10

Temperature, action of, 299
optimum, 298
pressure table, 500
range, 298
taking, 340

Test objects for objectives, 57

Testing filters, 478

Test-tubes, 3
to clean infected, 19
new, 18
to plug, 24
to sterilise, 31

Tetracocci, morphology of, 132

Thermal death-point, 143
determination of, 298
of spores, 301, 304
of vegetative forms, 298, 303

Thermophilic bacteria, 143

Thermo-regulators, Hearson's capsule, 218
Reichert's, 218

Thionine blue, 92

Thiothrix, morphology of, 133

Thresh's water collecting bottle, 418

Throttle pipettes, 13

Tinned meat, analysis of, 460

Tissue medium (Noguchi), 214
stains, 95

Tissues for sectioning, fixing, 114
freezing, 116
hardening, 114
imbedding, 118
preparation of, 114
washing, 115

Titration of media, 150

Torulae, differentiation from saccharomyces, 130

Total acidity, 280

Toxins, testing of, 318

Trephines, 360

Triple nosepiece, 58

True motility, 80

Tube cultures, preparation of, 222
length, 50

Tubercle bacillus in milk, 453
to stain, 110, 124

Tuberculous guinea-pig, cadaver of, 454

Tubing nutrient media, 160

Turnip media, 200

Unna-Pappenheim's stain for sections, 123

Unsound meat, analysis of, 460

Urine agar, 188
gelatine, 187
(Heller), 188
media bouillon, 187

Uschinsky's solution, 183

Valency of specific sera, 386

Van Ermengem's flagella stain, 104

Vegetative stage of bacteria, 136

Vesuvin, 94

Vibrio cholerae in milk, 452
in water, 439
morphology of, 133

Virulence, attenuating, 321
of organisms, 320
raising, 320

Vivisection license, 334

Voges holder, 350

Volatile oils as disinfectants, 27

Warm stage, 58

Washing red blood cells, 388
tissues, 115

Water, analysis of, qualitative, 426
quantitative, 416
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