dly becomes a homogenous but turbid
fluid--a process which may be hastened by:--

3. Placing the tube in the incubator at 37 deg. C. for 30 minutes--shaking
from time to time.

4. Centrifugalise the fluid thoroughly, at high speed.

5. Pipette off the supernatant fluid, fill up with sterile distilled
water, cork the tube and shake to distribute the deposit throughout the
water. Again centrifugalise.

6. Repeat steps 4 and 5 twice more.

7. Employ one portion of the final deposit to inoculate guinea pigs.

8. Plant the remainder of the deposit freely on Dorset's Egg medium; cap
and incubate at 37 deg. C.

NOTE.--If only microscopical films are needed, fill up the
centrifuge tube with Ligroin (a petroleum ether) in place of
sterile distilled water in step 5 and prepare the films from
the _surface_ of the fluid, to stain by the Ziehl-Neelsen
process.

INDEX

Abbe's condenser, 7

Abbott's stain for spores, 107

Aberration, chromatic, 56
spherical, 55

Absolute alcohol as a fixative, 82
as an antiseptic, 27

Absorbent paper for drying cover-slips, 69

A. C. E. mixture, 345

Acetic acid for clearing films, 82

Achromatic condenser, 54

Acid haematin, 96
production, analysis table, 283
by bacteria, 145
investigation of, 280
qualitative examination, 283, 284
quantitative examination, 280

Acid-fast bacilli in tissues, to stain, 124

Action of various gases on bacteria, 295

Active immunisation, illustrative example, 322

Adjustable water bath, 299

Aerobic cultures, 221

Aerogenic bacteria, 131

Aesculin agar, 204

Agar gelatine (guarniari), 194
methods of preparation, 167
surface plates, 232

Agar-agar, preparation of, 167

Agglutination reaction, macroscopical, 386
microscopical, 385

Agglutinin, 381

Air, analysis of, 468
filter, 40
pump, Geryk, 43

Albumin solution, Mayer's, 120

Alcohol production, test for, 285

Alkaline pyro, 239

Alum carmine, 96

Ammonia production test for, 285

Amphitrichous bacteria, 136

Anaerobic cultures, 236
Botkin's method, 243
Buchner's method, 238
Bulloch's method, 245
Hesse's method, 237
McLeod's method, 240
media, 180
Novy's method, 244

Anaerobic cultures, Roux's biological method, 237
physical method, 237
vacuum method, 238
Wright's method, 239

Anaesthetics, 345

Analysis of air, apparatus for, 469
method of, 468
qualitative bacteri