aesthetic, A. C. E._)

1. Anaesthetise the animal and secure it firmly to the operating table.

2. Irrigate the conjunctival sac thoroughly with sterile saline
solution.

3. Make an incision through the upper quadrant of the cornea into the
anterior chamber by means of a triangular keratome.

4. Separate the lips of the corneal wound with a flexible silver
spatula; seize the solid inoculum in a pair of iris forceps, introduce
it through the corneal wound, and deposit it on the anterior surface of
the iris; withdraw the forceps.

5. Again irrigate the sac and the surface of the cornea.

6. Release the animal from the operating table.

7. Label, etc.

~7. Intrapulmonary.~--

_Fluid Inoculum._--(_Anaesthetic, none._)

1. Have the animal firmly held by an assistant. (In this case the
foreleg of the selected side is drawn up by the assistant and held with
the ear of that side.)

2. Shave carefully in the axillary line and disinfect the denuded skin.

3. Thrust the needle of the syringe boldly through the fifth or sixth
intercostal space into the lung tissue.

4. Inject the contents of the syringe slowly.

5. Label, etc.

~8. Intravenous.~--

_Fluid Inoculum._--(_Anaesthetic, none._)

The site selected for the injection in the rabbit is the posterior
auricular vein (see Fig. 192). Although this is smaller than the median
vein, it is firmly bound down to the cartilage of the ear by dense
connective tissue, and is therefore more readily accessible. (In the
guinea-pig the jugular vein must be utilised, and in order to perform
the inoculation satisfactorily a general anaesthetic must be
administered to the animal. In the monkey or the dog, the internal
saphenous vein is the most convenient and before puncturing should be
distended or rendered prominent by compressing the vein above the
selected site.)

_Preparation of the Inoculum._--Care must be taken in preparing the
inoculum, as the injection of even small fragments may cause fatal
embolism. To obviate this risk the fluid should, if possible, be
filtered through sterile filter paper before filling into the syringe.

Air bubbles, when injected into a vein, frequently cause immediate
death. To prevent this, the syringe after being filled should be held in
the vertical position, needle uppermost. A piece of sterile filter paper
is then impaled on the needle and the piston of the syringe pressed
upward until all the air is expelled from the barrel and need