ass tube syringe for subcutaneous "solid"
inoculation.]

Or, improvise a syringe by sliding a piece of glass rod (to serve as a
piston) into the lumen of a slightly shorter length of glass tubing and
secure in position by a band of rubber tubing. Sterilise by boiling.
Withdraw the rod a few millimetres and deposit the piece of tissue
within the orifice of the tube, by means of sterile forceps. Now pass
the tube into the depths of the "pocket," push on the glass rod till it
projects beyond the end of the tube, and withdraw the apparatus, leaving
the tissue behind in the wound.

8. Close the wound in the skin with Michel's clips and a dressing of
gauze sealed with collodion (or Tinct. benzoin).

9. Label, etc.

~3. Intramuscular.~--

(a) _Fluid Inoculum.--(Anaesthetic, none.)_

Steps 1-4. As for cutaneous inoculation.

5. Steady the skin over the selected muscle or muscles with the slightly
separated left forefinger and thumb.

6. Thrust the needle of the injecting syringe boldly into the muscular
tissue and inject the inoculum slowly.

7. Label, etc.

(b) _Solid Inoculum.--(Anaesthetic, A. C. E.)_

1. Secure the animal to the operation table and anaesthetise.

2. Shave and disinfect the skin at the seat of operation.

3. Surround the field of operation by strips of gauze wrung out in 2 per
cent. lysol solution.

4. Incise skin, aponeurosis, and muscle in turn.

5. Deposit the inoculum in the depths of the incision.

6. Close the wound in the muscle with buried sutures and the cutaneous
wound with either continuous or interrupted sutures or with Michel's
steel clips.

7. Apply a sealed dressing of gauze and collodion.

8. Remove the animal from the operating table.

9. Label, etc.

~4. Intraperitoneal.~--

(a) _Fluid Inoculum.--(Anaesthetic, none.)_

Steps 1-4. As for cutaneous inoculation. Shave a fairly broad transverse
area, stretching from flank to flank.

5. Place the left forefinger on one flank and the thumb on the opposite,
and pinch up the entire thickness of the abdominal parietes in a
triangular fold. Now, by slipping the peritoneal surfaces (which are in
apposition) one over the other, ascertain that no coils of intestine are
included in the fold.

6. Take the syringe in the right hand and with the needle transfix the
fold near its base (Fig. 182).

7. Now release the fold, but hold the syringe steady; as the parietes
flatten out, the point of the needle is left free in the