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action, when it at once occurred to me that it was only natural that the air should be filtered of germs by the air passages, one of whose offices is to arrest inhaled particles of dust, and prevent them from entering the air-cells.' Here is a surmise which bears the stamp of genius, but which needs verification. If, for the words 'it is only natural' we were authorised to write 'it is perfectly certain,' the demonstration would be complete. Such demonstration is furnished by experiments with a beam of light. One evening, towards the close of 1869, while pouring various pure gases across the dusty track of a luminous beam, the thought occurred to me of using my breath instead of the gases. I then noticed, for the first time, the extraordinary darkness produced by the expired air, _towards the end of the expiration_. Permit me to repeat the experiment in your presence. I fill my lungs with ordinary air and breathe through a glass tube across the beam. The condensation of the aqueous vapour of the breath is shown by the formation of a luminous white cloud of delicate texture. We abolish this cloud by drying the breath previous to its entering the beam; or, still more simply, by warming the glass tube. The luminous track of the beam is for a time uninterrupted by the breath, because the dust returning from the lungs makes good, in great part, the particles displaced. After a time, however, an obscure disk appears in the beam, the darkness of which increases, until finally, towards the end of the expiration, the beam is, as it were, pierced by an intensely black hole, in which no particles whatever can be discerned. The deeper air of the lungs is thus proved to be absolutely free from suspended matter. It is therefore in the precise condition required by Professor Lister's explanation. This experiment may be repeated any number of times with the same result. I think it must be regarded as a crowning piece of evidence both of the correctness of Professor Lister's views and of the impotence, as regards vital development, of optically pure air. [Footnote: Dr. Burden Sanderson draws attention to the important observation of Brauell, which shows that the _contagium_ of a pregnant animal, suffering from splenic fever, is not found in the blood of the foetus; the placental apparatus acting as a filter, and holding back the infective particles.] Application of Luminous beams to Water. The method of examination h
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