FREE BOOKS

Author's List




PREV.   NEXT  
|<   182   183   184   185   186   187   188   189   190   191   192   193   194   195   196   197   198   199   200   201   202   203   204   205   206  
207   208   209   210   211   212   213   214   215   216   217   218   219   220   221   222   223   224   225   226   227   228   229   230   231   >>   >|  
enzymes. Enzymes are specific in their action. Any given enzyme affects only a single reaction; or at most acts only upon a single group of compounds which have similar molecular configuration. Usually it is only a single compound whose decomposition is accelerated by the action of a particular enzyme; but there are a few enzymes, such as _maltase_ (which acts on all [alpha]-glucosides) and emulsin (which acts on all [beta]-glucosides) which act catalytically upon groups of considerable numbers of similar compounds. Enzymes, like all other catalysts, act more energetically at increased temperatures; but for each particular enzyme there is an "optimum temperature," (usually between 40 deg. and 65 deg.) above which the destructive effect of the temperature upon the enzyme itself more than offsets the accelerating influence of the increased temperature. At still higher temperatures (usually 80 deg. to 100 deg.) the enzymes are "killed," i.e., rendered permanently inactive. All enzymes are "killed" by boiling the solutions in which they are contained. Dry preparations of enzyme material can withstand somewhat higher temperatures, for somewhat longer periods of time, than can the same enzyme in moist condition or in solution. When an enzyme has once been inactivated by heating, or "killed," it can never be restored to activity again. Enzymes are extremely sensitive to acids, bases, or salts, their activity being often enormously enhanced or, in other cases, entirely inhibited, by the presence in the reacting medium of very small amounts of free acids, or bases, or even of certain neutral salts. For example, pepsin, the enzyme of the stomach will act only in the presence of a slightly acid medium and is wholly inactive in a mixture which contains even the slightest amount of free alkaline material; while trypsin, the similar enzyme of the intestine, acts only under alkaline conditions. Practically all enzymes are rendered inactive, but not destroyed, by the presence of either acid or alkali in excess of N/10 strength. Many will act only in the presence of small quantities of certain specific neutral salts; while, on the other hand, other salts are powerful inhibitors of enzyme action. Enzymes often differ from the protoplasm which secretes them in their response to antiseptics, such as toluene, xylene, etc., which inhibit the activity or growth of the cell, but have no effect upon the activity of the enzymes which it con
PREV.   NEXT  
|<   182   183   184   185   186   187   188   189   190   191   192   193   194   195   196   197   198   199   200   201   202   203   204   205   206  
207   208   209   210   211   212   213   214   215   216   217   218   219   220   221   222   223   224   225   226   227   228   229   230   231   >>   >|  



Top keywords:
enzyme
 

enzymes

 

presence

 

activity

 

Enzymes

 

inactive

 

killed

 

action

 

temperatures

 
single

temperature

 

similar

 

alkaline

 

effect

 

material

 

medium

 

increased

 
rendered
 
neutral
 
higher

compounds

 

specific

 

glucosides

 

amounts

 

inhibit

 

xylene

 

response

 

antiseptics

 
toluene
 

reacting


excess
 
alkali
 

sensitive

 
growth
 
inhibited
 
enhanced
 

enormously

 

pepsin

 
trypsin
 
inhibitors

differ
 

amount

 

powerful

 
intestine
 
Practically
 

conditions

 

slightest

 

extremely

 

slightly

 

protoplasm