ege of Surgeons, England

Second Edition Rewritten and Enlarged

Philadelphia and London
W. B. Saunders Company
1913

Copyright, 1902, by W. B. Saunders and Company Revised, entirely
reset, reprinted, and recopyrighted July, 1913

Copyright, 1913, by W. B. Saunders Company

Registered at Stationers' Hall, London, England

Printed in America
Press of
W. B. Saunders Company
Philadelphia

TO THE MEMORY OF

JOHN WICHENFORD WASHBOURN, C.M.G., M.D., F.R.C.P.

Physician to Guy's Hospital and Lecturer on Bacteriology in the
Medical School, and Physician to the London Fever Hospital

MY TEACHER, FRIEND, AND CO-WORKER

PREFACE TO THE SECOND EDITION

Bacteriology is essentially a practical study, and even the elements of
its technique can only be taught by personal instruction in the
laboratory. This is a self-evident proposition that needs no emphasis,
yet I venture to believe that the former collection of tried and proved
methods has already been of some utility, not only to the student in the
absence of his teacher, but also to isolated workers in laboratories far
removed from centres of instruction, reminding them of forgotten details
in methods already acquired. If this assumption is based on fact no
further apology is needed for the present revised edition in which the
changes are chiefly in the nature of additions--rendered necessary by
the introduction of new methods during recent years.

I take this opportunity of expressing my deep sense of obligation to my
confrere in the Physiological Department of our medical school--Mr. J.
H. Ryffel, B. C., B. Sc.--who has revised those pages dealing with the
analysis of the metabolic products of bacterial life; to successive
colleagues in the Bacteriological Department of Guy's Hospital, for
their ready co-operation in working out or in testing new methods; and
finally to my Chief Laboratory Assistant, Mr. J. C. Turner whose
assistance and experience have been of the utmost value to me in the
preparation of this volume. I have also to thank Mrs. Constant Ponder
for many of the new line drawings and for redrawing a number of the
original cuts.

JOHN W. H. EYRE.

GUY'S HOSPITAL, S. E.
_July, 1913._

PREFACE TO THE FIRST EDITION

In the following pages I have endeavoured to arrange briefly and
concisely the various methods at present in use for the study of
bacteria, and the elucidation of such points in their lif