259

Scheme of Study, 259--Macroscopical
Examination of Cultivations,
261--Microscopical Methods,
272--Biochemical Methods, 276--Physical
Methods, 295--Inoculation Methods,
315--Immunisation, 321--Active
Immunisation, 322--The Preparation of
Haemolytic Serum, 327--The Titration of
Haemolytic Serum, 328--Storage of
Haemolysin, 331.

XVII. EXPERIMENTAL INOCULATION OF ANIMALS 332

Selection and Care of Animals,
335--Methods of Inoculation, 352.

XVIII. THE STUDY OF EXPERIMENTAL INFECTIONS DURING LIFE 370

General Observations, 371--Blood
Examinations, 373--Serological
Investigations, 378--Agglutinin,
381--Opsonin, 387--Immune Body, 393.

XIX. POST-MORTEM EXAMINATION OF EXPERIMENTAL ANIMALS 396

XX. THE STUDY OF THE PATHOGENIC BACTERIA 408

XXI. BACTERIOLOGICAL ANALYSES 415

Bacteriological Examination of Water,
416--Examination of Milk, 441--Ice Cream,
457--Examination of Cream and Butter,
457--Examination of Unsound Meats,
460--Examination of Oysters and Other
Shellfish, 463--Examination of Sewage and
Sewage Effluents, 466--Examination of
Air, 468--Examination of Soil,
470--Testing Filters, 478--Testing of
Disinfectants, 480.

APPENDIX 492

INDEX 505

[Illustration]

BACTERIOLOGICAL TECHNIQUE.

I. LABORATORY REGULATIONS.

The following regulations are laid down for observance in the
Bacteriological Laboratories under the direction of the author. Similar
regulations should be enforced in all laboratories where pathogenic
bacteria are studied.

_Guy's Hospital._

~BACTERIOLOGICAL DEPARTMENT.~

HANDLING OF INFECTIVE MATERIALS.

The following Regulations have been drawn up in the interest
of those working in the Laboratory as well as the public at
large, and will be strictly enforced.

Their object is to avoid the dangers of infection which may
arise from neglect of necessary precautions or from
carelessness.

Everyone must note that by neglecting the general rules laid
down he not only runs grave risk himself, but is a danger to
others.

REGULATIONS.

1. Each work