the work of Lehmann.

JOHN W. H. EYRE.

GUY'S HOSPITAL, S. E.

CONTENTS

PAGE

I. LABORATORY REGULATIONS 1

II. GLASS APPARATUS IN COMMON USE 3

The Selection, Preparation, and Care of
Glassware, 8--Cleaning of Glass
Apparatus, 18--Plugging Test-tubes and
Flasks, 24.

III. METHODS OF STERILISATION 26

Sterilising Agents, 26--Methods of
Application, 27--Electric Signal Timing
Clock, 38.

IV. THE MICROSCOPE 49

Essentials, 49--Accessories, 57--Methods
of Micrometry, 61.

V. MICROSCOPICAL EXAMINATION OF BACTERIA AND OTHER
MICRO-FUNGI 69

Apparatus and Reagents used in Ordinary
Microscopical Examination, 69--Methods of
Examination, 74.

VI. STAINING METHODS 90

Bacteria Stains, 90--Contrast Stains,
93--Tissue Stains, 95--Blood Stains,
97--Methods of Demonstrating Structure of
Bacteria, 99--Differential Methods of
Staining, 108.

VII. METHODS OF DEMONSTRATING BACTERIA IN TISSUES 114

Freezing Method, 115--Paraffin Method,
117--Special Staining Methods for
Sections, 121.

VIII. CLASSIFICATION OF FUNGI 126

Morphology of the Hyphomycetes,
126--Morphology of the Blastomycetes,
129.

IX. SCHIZOMYCETES 131

Anatomy, 134--Physiology,
136--Biochemistry, 144.

X. NUTRIENT MEDIA 146

Meat Extract, 148--Standardisation of
Media, 154--The Filtration of Media,
156--Storing Media in Bulk, 159--Tubing
Nutrient Media, 160.

XI. ORDINARY OR STOCK CULTURE MEDIA 163

XII. SPECIAL MEDIA 182

XIII. INCUBATORS 216

XIV. METHODS OF CULTIVATION 221

Aerobic, 222--Anaerobic, 236.

XV. METHODS OF ISOLATION 248

XVI. METHODS OF IDENTIFICATION AND STUDY