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fact of the splitting up of the sugar. What relation have these two facts to one another? For a very long time that was a great matter of dispute. The early French observers, to do them justice, discerned the real state of the case, namely, that there was a very close connection between the actual life of the yeast plant and this operation of the splitting up of the sugar; and that one was in some way or other connected with the other. All investigation subsequently has confirmed this original idea. It has been shown that if you take any measures by which other plants of like kind to the torula would be killed, and by which the yeast plant is killed, then the yeast loses its efficiency. But a capital experiment upon this subject was made by a very distinguished man, Helmholz, who performed an experiment of this kind. He had two vessels--one of them we will suppose full of yeast, but over the bottom of it, as this might be, was tied a thin film of bladder; consequently, through that thin film of bladder all the liquid parts of the yeast would go, but the solid parts would be stopped behind; the torula would be stopped, the liquid parts of the yeast would go. And then he took another vessel containing a fermentable solution of sugar, and he put one inside the other; and in this way you see the fluid parts of the yeast were able to pass through with the utmost ease into the sugar, but the solid parts could not get through at all. And he judged thus: if the fluid parts are those which excite fermentation, then, inasmuch as these are stopped, the sugar will not ferment; and the sugar did not ferment, showing quite clearly, that an immediate contact with the solid, living torula was absolutely necessary to excite this process of splitting up of the sugar. This experiment was quite conclusive as to this particular point, and has had very great fruits in other directions. Well, then, the yeast plant being essential to the production of fermentation, where does the yeast plant come from? Here, again, was another great problem opened up, for, as I said at starting, you have, under ordinary circumstances in warm weather, merely to expose some fluid containing a solution of sugar, or any form of syrup or vegetable juice to the air, in order, after a comparatively short time, to see all these phenomena of fermentation. Of course the first obvious suggestion is, that the torula has been generated within the fluid. In fact, it seem
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