re the remainder for future use.

~Egg-albumen (Tarchanoff and Kolesnikoff).~--

1. Place unbroken hens' eggs in dekanormal caustic soda solution for ten
days. (After this time the white becomes firm like gelatine.)

2. Carefully remove the shell and cut the egg into fine slices.

3. Wash for two hours in running water.

4. Place the egg slices in a large beaker and sterilise in the steamer
at 100 deg. C. for one hour.

5. Transfer each slice of egg by means of a pair of sterilised forceps
to a Petri dish or large capsule.

6. Sterilise in the steamer at 100 deg. C. for twenty minutes on each of
three consecutive days.

~Egg Albumin Broth (Lipschuetz).~--

1. Weigh out

Egg albumin (extra fine powder, Merck). 4 grammes

and place in a 2-litre flask with a number of sterile glass beads.

2. Measure out distilled water 200 c.c. into a half-litre flask and warm
to 37 deg. C. in the incubator.

3. Add the water to the flask containing the albumin and beads and
dissolve by shaking.

4. Add n/10-NaOH, 40 c.c. Allow the mixture to stand for thirty minutes
with frequent shaking.

5. Filter through Swedish filter paper.

6. Sterilise by boiling two or three times at intervals of two hours.

7. Add ordinary nutrient bouillon 600 c.c.

8. Fill into small Erlenmeyer flasks in quantities of 50 c.c.

9. Incubate for forty-eight hours at 37 deg. C.--discard any contaminated
flasks and store the remainder for future use.

~Egg Albumin Agar.~--

1. Prepare egg albumin solution as above 1-6.

2. Liquefy and measure out ordinary nutrient agar 600 c.c. and add to
the egg albumin solution (in place of the nutrient broth).

3. Complete as above 8-9.

_Diplococcus Meningitidis Intracellularis._

~Ascitic Fluid Agar (Wassermann)~ _Synonym_ ~N-as-gar (Mervyn Gordon).~

1. Liquefy and measure out into a sterile flask:

Nutrient agar 600 c.c.

2. Measure out into a half litre flask

Distilled water 210 c.c.

and add to it

Ascitic fluid 90 c.c.
Nutrose 6 grammes

3. Heat over a bunsen flame, shaking constantly until the fluid boils,
and the nutrose is dissolved.

4. Add the nutrose ascitic solution to the fluid agar.

5. Heat in the steamer for thirty minutes, then filter.

6. Tube and sterilise as for nutrient agar.

NOTE.--The finished medium in this case measures 900 c.c.
only since inconvenient fractions would be introduced in
making