51, k).

Stratiform: Liquefaction extending to the walls of the tube and downward
horizontally (Fig. 151, l).

(D) _Character of the Liquefied Gelatine._--

1. Pellicle on surface.

2. Uniformly turbid.

3. Granular.

4. Mainly clear, but containing flocculi.

5. Deposit at apex of liquefied portion.

(E) _Production of Gas Bubbles._

~Shake Cultures.~--

1. Presence or absence of liquefaction.

2. Production of gas bubbles.

3. Bulk of growth at the surface--aerobic.

4. Bulk of growth in depths--anaerobic.

~Fluid Media.~

~1. Surface of the Liquid.~--

Presence or absence of froth due to gas bubbles.

Presence or absence of pellicle formation.

Character of pellicle.

~2. Body of the Liquid.~--

Uniformly turbid.

Flocculi in suspension.

Granules in suspension.

Clear, with precipitate at bottom of tube.

Colouration of fluid, presence or absence of.

~3. Precipitate.~--

Character.

Amount.

Colour.

~Carbohydrate Media.~--

Growth.

Reaction.

Gas formation.

Coagulation or not of serum albumen (when serum water media are
employed).

~Litmus Milk Cultivations.~--

{Unaltered.
1. Reaction: {Acid.
{Alkaline.
2. Odour.

3. Formation of gas.

{Unaltered.
4. Consistency: {Peptonised (character of solution).
{Coagulated.

{hard: solid.
5. Clot: Character {soft: floculent.
{ragged and broken up by gas
{bubbles.

(a) Coagulum undissolved.

(b) Coagulum finally peptonised, completely: incompletely.

Resulting solution, clear: turbid.

{Abundant.
{Scanty.
6. Whey: {Clear.
{Turbid.
{Coagulated by boiling, or not.

~BY MICROSCOPICAL METHODS.~

As a council of perfection preparations must be made from pure
cultivations 4, 6, 8, 12, 18, and 24 hours; and subsequently at
intervals of, say, twenty-four hours, during the entire period they are
under observation, and examined--

(A) ~Living.--1.~ In ~hanging drop~, to determine _motility_ or
_non-motility_.

In this connection it must be remembered that under certain conditions
as to environment (e. g., when examined in an unsuitable medium,
atmosphere, temperature, etc.) motile bacilli may fail to exhibit
activity. No organism, therefore,