FREE BOOKS

Author's List




PREV.   NEXT  
|<   114   115   116   117   118   119   120   121   122   123   124   125   126   127   128   129   130   131   132   133   134   135   136   137   138  
139   140   141   142   143   144   145   146   147   148   149   150   151   152   153   154   155   156   157   158   159   160   161   162   163   >>   >|  
y passed urine in sterile flask. 2. Place the flask in the steamer at 100 deg. C. for thirty minutes. 3. Filter through two thicknesses of Swedish filter paper. 4. Tube, and sterilise as for nutrient bouillon. (Leave the reaction unaltered.) ~Urine Gelatine.~-- 1. Collect freshly passed urine in sterile flask. 2. Take the specific gravity, and, if above 1010, dilute with sterile water until that gravity is reached. 3. Estimate (with control) at the boiling-point, and note the reaction of the urine. 4. Weigh out gelatine, 10 per cent., and add to the urine in the flask. 5. Heat in the steamer at 100 deg. C. for one hour to dissolve the gelatine. 6. Estimate the reaction and add sufficient caustic soda solution to restore the reaction of the medium mass to the equivalent of the original urine. 7. Cool to 60 deg. C. and clarify with egg as for nutrient gelatine (_vide_ page 166). 8. Filter through papier Chardin. 9. Tube, and sterilise as for nutrient gelatine. ~Urine Gelatine (Heller).~-- 1. Collect freshly passed urine in sterile flask. 2. Filter through animal charcoal to remove part of the colouring matter. 3. Take the specific gravity, and if above 1010, dilute with sterile water till this gravity is reached. 4. Add Witte's peptone, 1 per cent.; salt, 0.5 per cent.; gelatine, 10 per cent. 5. Heat in the steamer at 100 deg. C. for one hour, to dissolve the gelatine, etc. 6. Add normal caustic soda solution in successive small quantities, and test the reaction from time to time with litmus paper, until the fluid reacts faintly alkaline. 7. Cool to 60 deg. C. and clarify with egg as for nutrient gelatine (_vide_ page 166). 8. Filter through papier Chardin. 9. Tube, and sterilise as for nutrient gelatine. ~Urine Agar.~-- 1. Collect freshly passed urine in sterile flask. 2. Take the specific gravity and if above 1010, dilute with sterile water till this gravity is reached. 3. Weigh out 1.5 per cent. or 2 per cent. powdered agar, and add it to the urine. 4. Heat in the steamer at 100 deg. C. for ninety minutes to dissolve the agar. 5. Cool to 60 deg. C. and clarify with egg as for nutrient agar (_vide_ page 168). 6. Filter through papier Chardin, using the hot-water funnel. 7. Tube, and sterilise as for nutrient agar. (Leave the reaction unaltered.) ~Serum Sugar Media (Hiss).~-- In these media the fermentation of carbohydrate substanc
PREV.   NEXT  
|<   114   115   116   117   118   119   120   121   122   123   124   125   126   127   128   129   130   131   132   133   134   135   136   137   138  
139   140   141   142   143   144   145   146   147   148   149   150   151   152   153   154   155   156   157   158   159   160   161   162   163   >>   >|  



Top keywords:
gelatine
 

sterile

 

nutrient

 
reaction
 

gravity

 

Filter

 

sterilise

 

passed

 

steamer

 

clarify


dilute

 
dissolve
 

reached

 
specific
 
Chardin
 

papier

 

freshly

 

Collect

 

solution

 

caustic


minutes

 

unaltered

 

Gelatine

 

Estimate

 

powdered

 
alkaline
 

reacts

 

quantities

 

litmus

 

faintly


fermentation

 

substanc

 
carbohydrate
 

successive

 

funnel

 

ninety

 

control

 

original

 

bouillon

 

Swedish


filter
 
equivalent
 

sufficient

 

boiling

 

medium

 
restore
 

thicknesses

 
Heller
 
peptone
 

thirty