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trace of alcohol. But forcibly submerge the little plant, push it down deep into the liquid, where the quantity of free oxygen that can reach it is insufficient for its needs, it immediately begins to act as a ferment, supplying itself with oxygen by the decomposition of the sugar, and producing alcohol as one of the results of the decomposition. Many other low microscopic plants act in a similar manner. In aerated liquids they flourish without any production of alcohol, but cut off from free oxygen they act as ferments, producing alcohol exactly as the real alcoholic leaven produces it, only less copiously. For the right apprehension of all these facts we are indebted to Pasteur. In the cases hitherto considered, the fermentation is proved to be the invariable correlative of life, being produced by organisms foreign to the fermentable substance. But the substance itself may also have within it, to some extent, the motive power of fermentation. The yeast-plant, as we have learned, is an assemblage of living cells; but so at bottom, as shown by Schleiden and Schwann, are all living organisms. Cherries, apples, peaches, pears, plums, and grapes, for example, are composed of cells, each of which is a living unit. And here I have to direct your attention to a point of extreme interest. In 1821, the celebrated French chemist, Berard, established the important fact that all ripening fruit, exposed to the free atmosphere, absorbed the oxygen of the atmosphere and liberated an approximately equal volume of carbonic acid. He also found that when ripe fruits were placed in a confined atmosphere, the oxygen of the atmosphere was first absorbed, and an equal volume of carbonic acid given out. But the process did not end here. After the oxygen had vanished, carbonic acid, in considerable quantities, continued to be exhaled by the fruits, which at the same time lost a portion of their sugar, becoming more acid to the taste, though the absolute quantity of acid was not augmented. This was an observation of capital importance, and Berard had the sagacity to remark that the process might be regarded as a kind of fermentation. Thus the living cells of fruits can absorb oxygen and breathe out carbonic acid, exactly like the living cells of the leaven of beer. Supposing the access of oxygen suddenly cut off, will the living fruit-cells as suddenly die, or will they continue to live as yeast lives, by extracting oxygen from
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