l plates of the first division, one
showing only the larger element of the heterochromosome pair (fig. 174,
_x_), the other both elements (fig. 173, _l_ and _s_). In the late
anaphase (fig. 175) the larger heterochromosome is often seen outside of
the polar mass, reminding one again of the "accessory" in the
Orthoptera. Occasionally it is found in some other isolated position
(fig. 176). Equatorial plates of the second division show the same
conditions as in the other species; some contain the larger
heterochromosome, others the smaller one (fig. 177, _a_ and _b_). It was
impossible to draw anaphases of the second division from a polar view
and the lateral view showed nothing unusual, merely the longitudinal
division of all of the chromosomes.
The spermatids show some interesting variations from the other species
which have been examined. In figures 178 and 179 we have telophases of
the second spermatocyte, showing centrosome and archoplasm (fig. 178)
and certain masses of deeply staining material in the cytoplasm (fig.
179, _a_{1}_). Figures 180 and 181 are young spermatids showing the
archoplasm from the second spindle (_a_{2}_) and a smaller, more deeply
staining mass (_a_{1}_), derived from the irregular masses of the
earlier stage (fig. 179, _a_{1}_). In figures 182 and 183, the axial
fiber has appeared and the larger mass of archoplasm (_a_{2}_) is being
transformed into a sheath. The other body remains unchanged. During the
following stages this smaller archoplasmic body (_a_{1}_) lies in close
contact with the axial fiber and sheath (_a_{2}_), and gradually
decreases in size (figs. 184-186) until it disappears in a slightly
later stage. The acrosome seems to develop directly out of the
cytoplasm. The enigmatical body (_a_{1}_), which is probably archoplasm
from the first maturation spindle, as it is not found in the cytoplasm
of the first spermatocyte, may serve as nutriment for the developing
axial fiber. The sperm head has a peculiar triangular form, staining
more deeply on two sides.
Miscellaneous Coleoptera.
Considerable material from the spruce borers was collected at Harpswell,
Maine, but the species were not identified. Although these insects were
in the pupa stage, most of the testes were too old. There were no
dividing spermatogonia and few spermatocyte mitoses. Most of the
spermatocytes contained 10 chromosomes, one of which was plainly an
unequal pair. In a few testes the number was 11, indica
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