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Project Gutenberg's Studies in Spermatogenesis, by Nettie Maria Stevens This eBook is for the use of anyone anywhere at no cost and with almost no restrictions whatsoever. You may copy it, give it away or re-use it under the terms of the Project Gutenberg License included with this eBook or online at www.gutenberg.net Title: Studies in Spermatogenesis Part II Author: Nettie Maria Stevens Release Date: March 7, 2010 [EBook #31546] Language: English Character set encoding: ASCII *** START OF THIS PROJECT GUTENBERG EBOOK STUDIES IN SPERMATOGENESIS *** Produced by Bryan Ness, Josephine Paolucci and the Online Distributed Proofreading Team at http://www.pgdp.net. (This file was produced from images generously made available by The Internet Archive/American Libraries.) STUDIES IN SPERMATOGENESIS PART II. (PAGES 33-74. PLATES VIII-XV.) A COMPARATIVE STUDY OF THE HETEROCHROMOSOMES IN CERTAIN SPECIES OF COLEOPTERA, HEMIPTERA AND LEPIDOPTERA, WITH ESPECIAL REFERENCE TO SEX DETERMINATION. BY N. M. STEVENS [Illustration] WASHINGTON, D. C.: Published by the Carnegie Institution of Washington October, 1906 CARNEGIE INSTITUTION OF WASHINGTON PUBLICATION NO. 36, PART II. FROM THE PRESS OF THE WILKENS-SHEIRY PRINTING CO. WASHINGTON, D. C. STUDIES IN SPERMATOGENESIS.--II. A COMPARATIVE STUDY OF THE HETEROCHROMOSOMES IN CERTAIN SPECIES OF COLEOPTERA, HEMIPTERA, AND LEPIDOPTERA, WITH ESPECIAL REFERENCE TO SEX DETERMINATION. By N. M. STEVENS. INTRODUCTION. In Part I of this series of papers, the spermatogenesis of five species belonging to four different orders of insects was considered. In two species of Orthoptera an "accessory chromosome" was found; in _Tenebrio molitor_, one of the Coleoptera, an unequal pair of chromosomes was described; in the other species no heterochromosomes were discovered. The apparent bearing of the chromosome conditions in _Tenebrio molitor_ on the problem of sex determination has led to a further investigation of the germ cells of the Coleoptera. One of the Hemiptera homoptera and two of the Lepidoptera have also been examined for comparison with the Coleoptera and the Hemiptera heteroptera. METHODS. As a result of previous experience with similar material, only two general methods of fixing and staining have been employed: (1) Fixation in Flemming's strong solution or Hermann's platino-aceto-osmic,
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