of a growing point with colchicine the outer layer of
cells may be doubled by colchicine but the lower layers may remain
unchanged. Or a reverse of this situation may obtain, and even other
types. Since the formation of pollen takes place from a certain layer it
is very important that such specific layers are changed. The course of
plant breeding can be altered by these kinds of changes. To our
knowledge, no investigations of periclinal chimeras have been made with
nuts, following treatment with colchicine.

Specific experiments were conducted at Eldred, Illinois in the spring of
1951 with seedlings of pecan. The cooperation of the R.B. Best Farms and
Nut Plantation made this project possible. Several types of treatment
were tried. Out of this experience the above schedule listed in 14 steps
was developed. Other details may be obtained by contacting the authors
direct. Observations of the new growth in 1951 and 1952 were made and
the shape of leaves, color, texture and general appearance suggest that
doubling of chromosomes has been induced. Up until the present time, no
microscopic analysis has been made but this is a contemplated step and
facilities are at hand to complete this work.

While this paper is not a completed research, the authors hope that the
presentation of technique will aid and stimulate interest in this new
approach to nut improvement. In such instances where certain members may
have a particular problem such as a true hybrid-sterile as a result of
hybridity, it is hoped that the suggestions given in the above pages may
lead into a new field of improvement. There are rewards in store for the
plant breeder willing to master this new technique, but the mastery
requires careful study and diligent work.

Literature Cited

1. Eigsti, O. J. and Dustin, P.--Colchicine Bibliography. Lloydia 10:
65-114. 1947.

2. ----, ----.--Colchicine Bibliography. Lloydia 12:185-207. 1949.

3. Dermen, H.--Ontogeny of tissues in stem and leaf of cytochimeral
apples. Am. Jour. Bot. 38:753-60. 1951.

4. Dermen, H. and Bain, H. F.--Periclinal and total polyploidy in
cranberries induced by colchicine. Proc. Am. Soc. Hort. Sci. 38:
400. 1941.

PRESIDENT MacDANIELS: The Resolutions Committee for this meeting is:
John Davidson, chairman, and Dr. Rohrbacher working with him. If you
have anything in mind that should be brought up in the resolutions, see
one of these two men.

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