of 13, and
all other New World hylids studied have _n_=12 (2_n_=24) (Duellman and
Cole, 1965; Duellman, 1967).

Cei (1963) and Cei and Erspamer (1966) noted that phyllomedusine frogs
differ notably from other Neotropical hylids on the basis of the amines
and polypeptides in the skin. All species of phyllomedusines deposit
their eggs in a gelatinous mass on leaves or branches above water.
Although this type of egg deposition is characteristic of some
rhacophorines and apparently all centrolenids, it is known among hylids
only in the phyllomedusines and in two species of _Hyla_.

The distinctive combination of morphological, physiological,
chromosomal, and behavioral characteristics is strongly suggestive that
these frogs represent an early phyletic divergence within the Hylidae.
Guenther (1859) proposed the familial name Phyllomedusidae for
_Phyllomedusa bicolor_ (Boddaert). I suggest the recognition of the
group as a subfamily. The following classification of the
phyllomedusines is based on my own knowledge of the Middle American and
some South American species and on evidence from the literature on those
South American species with which I am not personally familiar.

Subfamily Phyllomedusinae Guenther, 1859

Phyllomedusidae Guenther 1859 [Type genus, _Phyllomedusa_ Wagler,
1830].

_Definition._--Moderately small to large hylids having vertical pupils,
_n_=13 (2_n_=26) chromosomes, skin containing large amounts of
powerful bradykinin-like and physalaemin-like polypeptides, eggs
suspended from vegetation above water, and tadpoles have a ventral
spiracle sinistral to midline.

_Range._--Low and moderate elevations in South and Middle America,
including Trinidad, from northern Argentina and northwestern Ecuador to
Veracruz and southern Sonora, Mexico.

_Content._--Three genera, one of which probably is composite.

Genus =Agalychnis= Cope, 1864.

_Agalychnis_ Cope, 1864 [Type species, _Hyla moreletii_ Dumeril, 1853,
by subsequent designation].

_Definition._--Fingers and toes at least half webbed; terminal discs
large; first toe shorter than second and not opposable to others; skin
smooth, lacking osteoderms; parotoid glands, if present, poorly
developed and diffuse; palpebral membrane reticulate (except in _A.
calcarifer_); iris red or yellow; skull shallow, depth less than 40 per
cent of length; nasals large; frontoparietal fontanelle large;
quadratojugals reduced; prevomerine teeth present.