FREE BOOKS

Author's List




PREV.   NEXT  
|<   120   121   122   123   124   125   126   127   128   129   130   131   132   133   134   135   136   137   138   139   140   141   142   143   144  
145   146   147   148   149   150   151   152   153   154   155   156   157   158   159   160   161   162   163   164   165   166   167   168   169   >>   >|  
n of sodium carbonate, and add 5 drops of liquor pancreaticus, or a few grains of Fairchild's extract of pancreas, in each. Boil _B_, and make _C_ acid with dilute hydrochloric acid. Place in each tube an equal amount of well-washed fibrin, plug the tubes with absorbent cotton, and place all in a water-bath at about 100 degrees F. Experiment 73. Examine from time to time the three test tubes in the preceding experiment. At the end of one, two, or three hours, there is no change in _B_ and _C_, while in _A_ the fibrin is gradually being eroded, and finally disappears; but it does not swell up, and the solution at the same time becomes slightly turbid. After three hours, still no change is observable in _B_ and _C_. Experiment 74. Filter _A_, and carefully neutralize the filtrate with very dilute hydrochloric or acetic acid, equal to a precipitate of alkali-albumen. Filter off the precipitate, and on testing the filtrate, peptones are found. The intermediate bodies, the albumoses, are not nearly so readily obtained from pancreatic as from gastric digests. Experiment 75. Filter _B_ and _C_, and carefully neutralize the filtrates. They give no precipitate. No peptones are found. Experiment 76. _To show the action of pancreatic juice upon the albuminous ingredients (casein) of milk_. Into a four-ounce bottle put two tablespoonfuls of cold water; add one grain of Fairchild's extract of pancreas, and as much baking soda as can be taken up on the point of a penknife. Shake well, and add four tablespoonfuls of cold, fresh milk. Shake again. Now set the bottle into a basin of hot water (as hot as one can bear the hand in), and let it stand for about forty-five minutes. While the milk is digesting, take a small quantity of milk in a goblet, and stir in ten drops or more of vinegar. A thick curd of casein will be seen. Upon applying the same test to the digested milk, no curd will be made. This is because the pancreatic ferment (trypsin) has digested the casein into "peptone," which does not curdle. This digested milk is therefore called "peptonized milk." Experiment 77. _To show the action of bile_. Obtain from the butcher some ox bile. Note its bitter taste, peculiar odor, and greenish color. It is alkaline or neutral to litmus paper. Pour it from one vessel to another, and note that strings of mucin (from the lining membrane of the gall bladder)
PREV.   NEXT  
|<   120   121   122   123   124   125   126   127   128   129   130   131   132   133   134   135   136   137   138   139   140   141   142   143   144  
145   146   147   148   149   150   151   152   153   154   155   156   157   158   159   160   161   162   163   164   165   166   167   168   169   >>   >|  



Top keywords:
Experiment
 

digested

 

pancreatic

 

casein

 

precipitate

 
Filter
 

extract

 

change

 

pancreas

 

Fairchild


filtrate

 

peptones

 

neutralize

 

carefully

 
hydrochloric
 

tablespoonfuls

 

fibrin

 
dilute
 
bottle
 

action


goblet
 

quantity

 
bladder
 

digesting

 

lining

 

membrane

 

minutes

 

penknife

 

vessel

 

Obtain


butcher

 
bitter
 
alkaline
 

neutral

 

litmus

 

peculiar

 

greenish

 

peptonized

 

called

 

applying


strings

 

vinegar

 

curdle

 

peptone

 
ferment
 

trypsin

 

Examine

 
preceding
 
experiment
 

degrees