sely Gram-positive;
in succeeding stages the irregular, vacuolated, inflated, and ruptured
forms predominate. Between the eighteenth and twenty-fourth hours of
incubation at 112 deg. F. the strains of type A develop oval to
kidney-shaped nodules attached to a stem extending from the cell
substance. As the incubation is prolonged these nodules increase in
size, often measuring 1 mu to 2 mu in length; this nodule formation occurs
at the expense of the cell protoplasm, and appears to be a marked
characteristic of growth in whey. Cultures of type B do not form nodules
or clubs, but small spherical bodies more or less securely attached to
the cell wall are seen. Again, type A assumes the form of small bacilli
in chains, while type B strains develop to a greater length and exist
almost exclusively as single isolated forms. True branching has been
observed in strains of type B.

_In Milk._--In milk there is a tendency to thread-formation consisting
of four to ten segments in the case of type A, while type B shows longer
and more curved forms. With increasing age of the culture there also
appears to be increase in the length of the organisms. All strains are
non-motile, non-sporogenous, and non-capsule-forming.

_Staining Reactions._--All strains are readily stained by the usual
aniline dyes.

_A. Gram's Method._--Young individuals give an intense reaction with
this stain; old bacilli are easily decolourised, and degenerate forms
are always Gram-negative, while single individuals have been observed
which showed gradation from one pole of the cell to the other.

_B. Loeffler's Methylene Blue._--According to the behaviour of the
organisms studied, a separation into two types appears possible, type A
being uniformly impregnated, while type B shows distinct
differentiation. The cell body is seen to contain a varying number of
round to oval bodies or granules. This is the appearance already
mentioned by Dueggeli, Luerssen and Kuehn, and Kuntze, and from which the
granule bacillus derives its name. In opposition to the observations of
Kuntze, the occurrence of granules was not found to be variable; it was,
indeed, so constant as to constitute a distinguishing characteristic
between the two types. The organisms of this group are difficult to
cultivate, and freshly isolated growth is obtainable only on media
containing whey, malt, or in milk. They grow equally well under aerobic
or anaerobic conditions. The optimum temperature fo