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<![CDATA[ 1.0 gramme
and dissolve in
Distilled water 100.0 c.c.
Filter.
~Vesuvin (or Bismarck Brown).~--
17. _Saturated Aqueous Solution._
Weigh out
Vesuvin 0.5 gramme
and dissolve in
Distilled water 100.0 c.c.
Filter.
TISSUE STAINS.
~Aniline Gentian Violet~ (For Weigert's Fibrin Stain).--
Weigh out
Gentian violet 1.0 gramme
and dissolve in
Absolute alcohol 15.0 c.c.
Distilled water 80.0 c.c.
then add
Aniline oil 3.0 c.c.
Shake well and filter before use.
~Haematoxylin~ (Ehrlich).--
1. Weigh out
Haematoxylin 2.0 grammes
and dissolve in
Absolute alcohol 100.0 c.c.
2. Weigh out
Ammonium alum 2.0 grammes
and dissolve in
Distilled water 100.0 c.c.
3. Mix 1 and 2, allow the mixture to stand forty-eight hours, then
filter.
4. Add
Glycerine 85.0 c.c.
Acetic acid, glacial 10.0 c.c.
5. Allow the stain to stand for one month exposed to light; then filter
again ready for use.
~Haematin~ (Mayer's).--
A. Weigh out
Haematin 1.0 gramme
and dissolve in
Alcohol 90 per cent. (warmed to 37 deg. C.) 50 c.c.
B. Weigh out
Potash alum 50 grammes
and dissolve in
Distilled water 100 c.c.
Prepare these two solutions in separate flasks. Take a clean flask of
250 c.c. capacity and insert a large funnel in its neck. Pour the
solutions A and B simultaneously and slowly into the funnel to mix
thoroughly. Store for future use.
NOTE.--If acid haematin is required, introduce glacial acetic
acid (3 c.c.) into the mixing flask before adding the
solutions A and B.
~Alum Carmine~ (Mayer).--
Weigh out
Alum 2.5 grammes
Carmine 1.0 gramme
and place in a glass beaker.
Measure out in a measuring cylinder,
Distilled water 100.0 c.c.
Place the beaker on a sand-bath, add the water in successive small
quantities, and keep the mixture boiling for twenty minutes. Measure the
solution and make up to 100 c.c. by the addition of distilled water.
Filter.
~Lithium Carmine~ (Orth).--
Weigh out
Carmine 2.5 grammes
and dissolve in
Lithium carbonate, cold saturated solution 100.0 c.c.
Filter.
~Picrocarmine.~--
Weigh out
Picrocarmine 2.0 ]]>
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