, under water and made a hole in the bladder, when two
ounces of water rose into it. This small diminution of the air was
probably caused by the heat which the rat took with it, which had
previously driven the air out.

+84. Second Experiment.+--I took a large soft bladder and fastened a
tube into its opening; then I filled it with the air out of my lungs,
and held the tube and bladder with my right hand and closed my nostrils
with the left. I respired the air as long as I could, and was able to
make 24 inspirations (regarding which it is to be observed that at the
last I was obliged to draw the whole bladder full of air into my lungs
at once, while at the beginning only the half of it was necessary). I
then closed the tube with my finger, and tied up the bladder. This air
had properties similar to the preceding in which the rat died. That is
to say, it contained one-thirtieth part of aerial acid, which I
separated from it by milk of lime; and a burning candle at once went out
in it.

+85. Third Experiment.+--I placed a few flies in a bottle into which I
had put some honey smeared upon paper. After a few days they had died.
They likewise had not absorbed any air; milk of lime, however,
diminished this air about one fourth part, and the remainder
extinguished fire.

I then took a bottle of 20 ounces measure and bored a hole in the bottom
of it with the corner of a broken file (Fig. 5, A). Into this bottle I
put a small piece of unslaked lime, and closed the mouth with a cork
through which I had previously fixed a tube B. Round about this cork I
placed a ring of pitch, and placed over it an inverted glass C, into
which I had previously put a large bee and had given it some honey which
was smeared upon paper; but in order that no air could penetrate within
the ring of pitch, I pressed the glass firmly in; I afterwards placed
the bottle in the dish D, into which I poured so much water that it was
half immersed in it; as soon I observed that the bottle was raised by
the water, I put a small weight upon the glass. The water rose a little
into the bottle every day through the opening A; and I also shook the
bottle a little sometimes in order that the skin which formed over the
milk of lime might break. After the lapse of seven days the water had
risen to E, and the bee was dead. Occasionally I put 2 bees into the
glass C, when just as much air was converted into aerial acid in half
the time. Caterpillars and butterflies