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ptivity is longer than in the Japanese and hybrid chestnuts tested. They maintained full receptivity on the 22nd day after the beginning of anthesis. It is commonly believed that bagging as well as emasculation may seriously affect the yield from controlled pollination. This is not always the case. One of the Japanese trees and one hybrid tree (S8 x J) yielded fully as many nuts from controlled (under best conditions) as from open pollination. On all other trees the effect of bagging was more or less adverse. The Effect of Emasculation on Nut Yield Emasculation involves the removal of the unisexual male catkins and the male part of the bisexual catkins. In the course of the controlled pollination work it has often been found that the female flowers drop off in the bag before the burs start to develop. This has especially been encountered in Japanese x American hybrids and back-crosses. It was thought that this perhaps was due to injuries resulting from emasculation. The following small experiment was carried out in order to determine if this was actually true. A Japanese x American and a Japanese x (Japanese x American) hybrid were used as the female parents. On these trees some flowering branches were bagged which had been emasculated normally, on other branches only the unisexual catkins were removed, while the bisexual catkins were left intact. Some branches were bagged without any emasculation, and some flowering branches were just tagged. The number of female flowers was counted in all cases. Pollinations were performed 3 times, that is, were repeated on the third and fifth day after the first pollination. This is the procedure ordinarily used for our controlled pollinations. Chinese pollen was used on both trees. Nut set expressed as per cent of the number of pollinated flowers, times three, (because ordinarily there are 3 nuts in every bur) was taken as a measure of how successful the pollinations had been. The results are shown in Table 2. Table II. The Effect of Emasculation on Nut Yield ---------------------------------------------------------------------------------------- Type of Treatment No. of [Symbol: female]'s at Nut set as time of first expressed in % pollination of [Symbol: female]'s at time
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